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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APH1A Polyclonal antibody (11643-1-AP)

    WB analysis using 11643-1-AP (same clone as 11643-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using APH1A Polyclonal antibody (11643-1-AP)

    WB analysis using 11643-1-AP (same clone as 11643-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using APH1A Polyclonal antibody (11643-1-AP)

    WB analysis of HeLa using 11643-1-AP (same clone as 11643-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using APH1A Polyclonal antibody (11643-1-AP)

    WB analysis of human brain using 11643-1-AP (same clone as 11643-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using APH1A Polyclonal antibody (11643-1-AP)

    IP experiment of HeLa using 11643-1-AP (same clone as 11643-1-PBS)

    IP result of anti-APH1A (IP:11643-1-AP, 4ug; Detection:11643-1-AP 1:4000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using APH1A Polyclonal antibody (11643-1-AP)

    IHC staining of human stomach using 11643-1-AP (same clone as 11643-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11643-1-AP (APH1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using APH1A Polyclonal antibody (11643-1-AP)

    IHC staining of human stomach using 11643-1-AP (same clone as 11643-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11643-1-AP (APH1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using APH1A Polyclonal antibody (11643-1-AP)

    IF Staining of HeLa using 11643-1-AP (same clone as 11643-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APH1A antibody (11643-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11643-1-PBS in a different storage buffer formulation.

APH1A Polyclonal antibody, PBS Only

APH1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11643-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

APH 1A, Aph 1alpha, APH-1a, Aph-1alpha, CGI 78

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11643-1-PBS targets APH1A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2238

Product name: Recombinant human APH1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-265 aa of BC015568

Sequence: MGAAVFFGCTFVAFGPAFALFLITVAGDPLRVIILVAGAFFWLVSLLLASVVWFILVHVTDRSDARLQYGLLIFGAAVSVLLQEVFRFAYYKLLKKADEGLASLSEDGRSPISIRQMAYVSGLSFGIISGVFSVINILADALGPGVVGIHGDSPYYFLTSAFLTAAIILLHTFWGVVFFDACERRRYWALGLVVGSHLLTSGLTFLNPWYEASLLPIYAVTVSMGLWAFITAGGSLRSIQRSLLCRRQEDSRVMVYSALRIPPED

相同性解析による交差性が予測される生物種
Full Name anterior pharynx defective 1 homolog A (C. elegans)
Calculated molecular weight 265 aa, 29 kDa
Observed molecular weight27-32 kDa
GenBank accession numberBC015568
Gene Symbol APH1A
Gene ID (NCBI) 51107
RRIDAB_2242861
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BI3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

APH1A is a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease

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