APH1A Polyclonal antibody

Name: APH1A Polyclonal antibody
Brand: Proteintech
SKU: 11643-1-AP

APH1A Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11643-1-AP

Synonyms

6530402N02Rik, APH 1A, Aph 1alpha, APH1A, CGI 78, Gamma secretase subunit APH 1A, PSF, UNQ579/PRO1141

Validation Data Gallery

Applications:

AllWBIP

WB analysis using 11643-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 11643-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11643-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11643-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11643-1-AP
IP result of anti-APH1A (IP:11643-1-AP, 4ug; Detection:11643-1-AP 1:4000) with HeLa cells lysate 1600 ug.

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Cat No. 11643-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HT-29 cells, HeLa cells, human brain tissue, U-937 cells, rat brain tissue
Positive IP detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 3 publications below

Product Information

11643-1-AP targets APH1A in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	APH1A fusion protein Ag2238 相同性解析による交差性が予測される生物種
Full Name	anterior pharynx defective 1 homolog A (C. elegans)
Calculated molecular weight	265 aa, 29 kDa
Observed molecular weight	27-32 kDa
GenBank accession number	BC015568
Gene symbol	APH1A
Gene ID (NCBI)	51107
RRID	AB_2242861
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for APH1A antibody 11643-1-AP	Download protocol
IP protocol for APH1A antibody 11643-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Neurobiol Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression. Authors - Yasuteru Inoue View Article
human	WB	Front Neurosci Impacts of Acute Hypoxia on Alzheimer's Disease-Like Pathologies in APPswe/PS1dE9 Mice and Their Wild Type Littermates. Authors - Feng Zhang View Article
mouse	WB	Neurosci Lett Metformin attenuates vascular pathology by increasing expression of insulin-degrading enzyme in a mixed model of cerebral amyloid angiopathy and type 2 diabetes mellitus. Authors - Yasuteru Inoue View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APH1A Polyclonal antibody

APH1A Polyclonal Antibody for IP, WB, ELISA

Validation Data Gallery

WB analysis using 11643-1-AP

WB analysis using 11643-1-AP

WB analysis of HeLa using 11643-1-AP

WB analysis of human brain using 11643-1-AP

IP experiment of HeLa using 11643-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.

Impacts of Acute Hypoxia on Alzheimer's Disease-Like Pathologies in APPswe/PS1dE9 Mice and Their Wild Type Littermates.

Metformin attenuates vascular pathology by increasing expression of insulin-degrading enzyme in a mixed model of cerebral amyloid angiopathy and type 2 diabetes mellitus.