Various lysates were subjected to SDS PAGE followed by western blot with 83731-2-RR (ACOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83731-2-PBS in a different storage buffer formulation.

K-562 and U-251 cell lysates were measured. The human AOX concentration of detected samples was determined to be 158.79 ng/mL (based on a 2.1 mg/mL extract load) in K-562 cell lyaste and 345.46 ng/mL (based on a 1.6 mg/mL extract load) in U-251 cell lysate.

Sandwich ELISA standard curve of MP00709-3, Human AOX Recombinant Matched Antibody Pair - PBS only. 83731-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1405. 83731-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83731-2-RR (ACOX1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83731-2-PBS in a different storage buffer formulation.

1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX1 Recombinant antibody (83731-2-RR, Clone:240754E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83731-2-PBS in a different storage buffer formulation.

Cytometric bead array standard curve of MP00709-3, AOX Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83731-1-PBS. Detection antibody: 83731-2-PBS. Standard: Ag1405. Range: 0.78-50 ng/mL.

Biolayer interferometry (BLl) kinetic assays of 83731-2-RR against Human ACOX1 were performed. The affinity constant is below 1 pM.