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Validation Data Gallery

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  • Western Blot (WB) analysis of rat testis tissue using ANKRD31 Polyclonal antibody (29891-1-AP)

    WB analysis of rat testis using 29891-1-AP (same clone as 29891-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 29891-1-AP (ANKRD31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29891-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ANKRD31 Polyclonal antibody (29891-1-AP)

    WB analysis using 29891-1-AP (same clone as 29891-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29891-1-AP (ANKRD31 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29891-1-PBS in a different storage buffer formulation.

ANKRD31 Polyclonal antibody, PBS Only

ANKRD31 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 29891-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29891-1-PBS targets ANKRD31 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31858

Product name: Recombinant human ANKRD31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of NM_001164443.1

Sequence: MEEGVQAPDWDSDETVIEGSVTESDLEEKELPWRRLLFDQDASLKSEFSLHPDTRGMCKGMPSPEIQLGFKLREDLQEQMNKNKMMPVLSEDTILQSQDETERNQALLQTRKNCSMFIGSFRQSGLSLNHQNIEGPEAESPEVLPHIEKELSEGRDSPEVSLLSGTAITVSDTVAVKETSLVEPEKILAAPNTFFEPRKE

相同性解析による交差性が予測される生物種
Full Name ankyrin repeat domain 31
Calculated molecular weight211 kDa
Observed molecular weight210 kDa
GenBank accession numberNM_001164443.1
Gene Symbol ANKRD31
Gene ID (NCBI) 256006
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N7Z5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ANKRD31 (Ankyrin repeat domain-containing protein 31) controls the number, timing, and location of meiotic DNA breaks during meiosis. It encodes a 1857 amino acid protein with 2 ankyrin repeat domains (PubMed: 31003867). ANKRD31 was a meiotic protein colocalized REC114 on chromatin (PubMed: 31000436). Mutations in ANKRD31 are associated with a Rett syndrome (RTT)-like phenotype (PMID: 27541642). And ANKRD31 has a high expression in testis.

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