Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat testis tissue, mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29891-1-AP targets ANKRD31 in WB, ELISA applications and shows reactivity with Rat, mouse, human samples.
| Tested Reactivity | Rat, mouse, human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ANKRD31 fusion protein Ag31858 相同性解析による交差性が予測される生物種 |
| Full Name | ankyrin repeat domain 31 |
| Calculated molecular weight | 211 kDa |
| Observed molecular weight | 210 kDa |
| GenBank accession number | NM_001164443.1 |
| Gene Symbol | ANKRD31 |
| Gene ID (NCBI) | 256006 |
| RRID | AB_3086181 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N7Z5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ANKRD31 (Ankyrin repeat domain-containing protein 31) controls the number, timing, and location of meiotic DNA breaks during meiosis. It encodes a 1857 amino acid protein with 2 ankyrin repeat domains (PubMed: 31003867). ANKRD31 was a meiotic protein colocalized REC114 on chromatin (PubMed: 31000436). Mutations in ANKRD31 are associated with a Rett syndrome (RTT)-like phenotype (PMID: 27541642). And ANKRD31 has a high expression in testis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ANKRD31 antibody 29891-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |