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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AMPK Alpha Monoclonal antibody

AMPK Alpha Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (1)

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8E1

Cat no : CL488-66536

Synonyms

AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1

Validation Data Gallery

Applications:
  • IF Staining of human breast cancer using CL488-66536

    IF Staining of human breast cancer using CL488-66536

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-66536 (AMPK alpha antibody) at dilution of 1:100.

  • IF Staining of human breast cancer using CL488-66536

    IF Staining of human breast cancer using CL488-66536

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-66536 (AMPK alpha antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy PRKAA1 Ready-To-Use IHC Kit. PRKAA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66536-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

CL488-66536 targets AMPK Alpha in IF applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AMPK Alpha fusion protein Ag24744 相同性解析による交差性が予測される生物種
Full Name protein kinase, AMP-activated, alpha 1 catalytic subunit
Calculated molecular weight 63 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC012622
Gene symbol PRKAA1
Gene ID (NCBI) 5562
RRIDAB_2883352
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 AMPK Alpha antibody CL488-66536Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Rep

Downregulation of hepatic ceruloplasmin ameliorates NAFLD via SCO1-AMPK-LKB1 complex

Authors - Liping Xie
View Article