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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using AMMECR1 Polyclonal antibody (24687-1-AP)

    WB analysis of Jurkat using 24687-1-AP (same clone as 24687-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24687-1-AP (AMMECR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using AMMECR1 Polyclonal antibody (24687-1-AP)

    WB analysis of mouse liver using 24687-1-AP (same clone as 24687-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24687-1-AP (AMMECR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse uterus tissue using AMMECR1 Polyclonal antibody (24687-1-AP)

    WB analysis of mouse uterus using 24687-1-AP (same clone as 24687-1-PBS)

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 24687-1-AP (AMMECR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24687-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using AMMECR1 Polyclonal antibody (24687-1-AP)

    IHC staining of mouse cerebellum using 24687-1-AP (same clone as 24687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24687-1-AP (AMMECR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24687-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using AMMECR1 Polyclonal antibody (24687-1-AP)

    IHC staining of mouse cerebellum using 24687-1-AP (same clone as 24687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24687-1-AP (AMMECR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24687-1-PBS in a different storage buffer formulation.

AMMECR1 Polyclonal antibody, PBS Only

AMMECR1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24687-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24687-1-PBS targets AMMECR1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16977

Product name: Recombinant human AMMECR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-296 aa of BC060813

Sequence: MAAGCCGVKKQKLSSSPPSGSGGGGGASSSSHCSGESQCRAGELGLGGAGTRLNGLGGLTGGGSGSGCTLSPPQGCGGGGGGIALSPPPSCGVGTLLSTPAAATSSSPSSSSAASSSSPGSRKMVVSAEMCCFCFDVLYCHLYGYQQPRTPRFTNEPYALKDSRFPPMTRDELPRLFCSVSLLTNFEDVCDYLDWEVGVHGIRIEFINEKGSKRTATYLPEVAKEQGWDHIQTIDSLLRKGGYKAPITNEFRKTIKLTRYRSEKMTLSYAEYLAHRQHHHFQNGIGHPLPPYNHYS

相同性解析による交差性が予測される生物種
Full Name Alport syndrome, mental retardation, midface hypoplasia and elliptocytosis chromosomal region gene 1
Calculated molecular weight 330 aa, 35 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC060813
Gene Symbol AMMECR1
Gene ID (NCBI) 9949
RRIDAB_2879671
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4X0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AMMECR1 also named as AMME syndrome candidate gene 1 protein is a 333 amino acid protein, which contains 1 AMMECER1 domain. Containing a glycine-rich N terminus, the AMMECR1 protein exhibits putative nuclear localization and a substantial level of instability, suggesting it plays a role in regulation. The deletion of AMMECR1 gene may be involved in glomerulonephritis, sensorineural hearing loss, mental retardation, midface hypoplasia and elliptocytosis.

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