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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using AMMECR1 Polyclonal antibody (24687-1-AP)

    WB analysis of Jurkat using 24687-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24687-1-AP (AMMECR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using AMMECR1 Polyclonal antibody (24687-1-AP)

    WB analysis of mouse liver using 24687-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24687-1-AP (AMMECR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse uterus tissue using AMMECR1 Polyclonal antibody (24687-1-AP)

    WB analysis of mouse uterus using 24687-1-AP

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 24687-1-AP (AMMECR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using AMMECR1 Polyclonal antibody (24687-1-AP)

    IHC staining of mouse cerebellum using 24687-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24687-1-AP (AMMECR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using AMMECR1 Polyclonal antibody (24687-1-AP)

    IHC staining of mouse cerebellum using 24687-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24687-1-AP (AMMECR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AMMECR1 Polyclonal antibody

AMMECR1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24687-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

AMMECR1, AMMERC1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, mouse liver tissue, mouse uterus tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24687-1-AP targets AMMECR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16977

Product name: Recombinant human AMMECR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-296 aa of BC060813

Sequence: MAAGCCGVKKQKLSSSPPSGSGGGGGASSSSHCSGESQCRAGELGLGGAGTRLNGLGGLTGGGSGSGCTLSPPQGCGGGGGGIALSPPPSCGVGTLLSTPAAATSSSPSSSSAASSSSPGSRKMVVSAEMCCFCFDVLYCHLYGYQQPRTPRFTNEPYALKDSRFPPMTRDELPRLFCSVSLLTNFEDVCDYLDWEVGVHGIRIEFINEKGSKRTATYLPEVAKEQGWDHIQTIDSLLRKGGYKAPITNEFRKTIKLTRYRSEKMTLSYAEYLAHRQHHHFQNGIGHPLPPYNHYS

相同性解析による交差性が予測される生物種
Full Name Alport syndrome, mental retardation, midface hypoplasia and elliptocytosis chromosomal region gene 1
Calculated molecular weight 330 aa, 35 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC060813
Gene Symbol AMMECR1
Gene ID (NCBI) 9949
RRIDAB_2879671
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4X0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AMMECR1 also named as AMME syndrome candidate gene 1 protein is a 333 amino acid protein, which contains 1 AMMECER1 domain. Containing a glycine-rich N terminus, the AMMECR1 protein exhibits putative nuclear localization and a substantial level of instability, suggesting it plays a role in regulation. The deletion of AMMECR1 gene may be involved in glomerulonephritis, sensorineural hearing loss, mental retardation, midface hypoplasia and elliptocytosis.

Protocols

Product Specific Protocols
IHC protocol for AMMECR1 antibody 24687-1-APDownload protocol
WB protocol for AMMECR1 antibody 24687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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