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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using ALG5 Polyclonal antibody (16046-1-AP)

    WB analysis of human kidney using 16046-1-AP (same clone as 16046-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ALG5 Polyclonal antibody (16046-1-AP)

    WB analysis of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ALG5 Polyclonal antibody (16046-1-AP)

    IHC staining of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ALG5 Polyclonal antibody (16046-1-AP)

    IHC staining of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALG5 Polyclonal antibody (16046-1-AP)

    IHC staining of human liver cancer using 16046-1-AP (same clone as 16046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALG5 Polyclonal antibody (16046-1-AP)

    IHC staining of human liver cancer using 16046-1-AP (same clone as 16046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ALG5 Polyclonal antibody (16046-1-AP)

    IHC staining of human placenta using 16046-1-AP (same clone as 16046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16046-1-AP (ALG5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ALG5 Polyclonal antibody (16046-1-AP)

    IHC staining of human placenta using 16046-1-AP (same clone as 16046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16046-1-AP (ALG5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ALG5 Polyclonal antibody (16046-1-AP)

    IF Staining of HEK-293 using 16046-1-AP (same clone as 16046-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 16046-1-AP (ALG5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

ALG5 Polyclonal antibody, PBS Only

ALG5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16046-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16046-1-PBS targets ALG5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8990

Product name: Recombinant human ALG5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 34-324 aa of BC012531

Sequence: MPALHRHEEEKFFLNAKGQKETLPSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALSYLEKRQKRDPAFTYEVIVVDDGSKDQTSKVAFKYCQKYGSDKVRVITLVKNRGKGGAIRMGIFSSRGEKILMADADGATKFPDVEKLEKGLNDLQPWPNQMAIACGSRAHLEKESIAQRSYFRTLLMYGFHFLVWFLCVKGIRDTQCGFKLFTREAASRTFSSLHVERWAFDVELLYIAQFFKIPIAEIAVNWTEIEGSKLVPFWSWLQMGKDLLFIRLRYLTGAWRLEQTRKMN

相同性解析による交差性が予測される生物種
Full Name asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferase homolog (S. cerevisiae)
Calculated molecular weight 324 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC012531
Gene Symbol ALG5
Gene ID (NCBI) 29880
RRIDAB_2273966
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y673
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Dolichyl-phosphate beta-glucosyltransferase(ALG5), belongs to the family of glycosyltransferases, specifically the hexosyltransferases. ALG5 is widely expressed in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung. ALG5 can catalyze the chemical reaction between UDP-glucose and UDP with dolichyl phosphate.

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