• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALG5 Polyclonal antibody

ALG5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16046-1-AP

Synonyms

ALG5, DolP glucosyltransferase, RP11 421P11.2

Validation Data Gallery

Applications:
  • WB analysis of human kidney using 16046-1-AP

    WB analysis of human kidney using 16046-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 16046-1-AP

    WB analysis of mouse liver using 16046-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 16046-1-AP

    IHC staining of mouse liver using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 16046-1-AP

    IHC staining of mouse liver using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 16046-1-AP

    IHC staining of human liver cancer using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human liver cancer using 16046-1-AP

    IHC staining of human liver cancer using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human placenta using 16046-1-AP

    IHC staining of human placenta using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16046-1-AP (ALG5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 16046-1-AP

    IHC staining of human placenta using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16046-1-AP (ALG5 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HEK-293 using 16046-1-AP

    IF Staining of HEK-293 using 16046-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 16046-1-AP (ALG5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman kidney tissue, mouse liver tissue
Positive IHC detected inmouse liver tissue, human liver cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16046-1-AP targets ALG5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALG5 fusion protein Ag8990 相同性解析による交差性が予測される生物種
Full Name asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferase homolog (S. cerevisiae)
Calculated molecular weight 324 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC012531
Gene symbol ALG5
Gene ID (NCBI) 29880
RRIDAB_2273966
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dolichyl-phosphate beta-glucosyltransferase(ALG5), belongs to the family of glycosyltransferases, specifically the hexosyltransferases. ALG5 is widely expressed in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung. ALG5 can catalyze the chemical reaction between UDP-glucose and UDP with dolichyl phosphate.

Protocols

Product Specific Protocols
WB protocol for ALG5 antibody 16046-1-APDownload protocol
IHC protocol for ALG5 antibody 16046-1-APDownload protocol
IF protocol for ALG5 antibody 16046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols