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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated ALDH9A1 Monoclonal a (CL488-67372)

    IF Staining of HeLa using CL488-67372

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ALDH9A1 antibody (CL488-67372, Clone: 3C5C9 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated ALDH9A1 Monoclonal antibody

ALDH9A1 Monoclonal Antibody for IF/ICC
Cat No. CL488-67372
Clone No.3C5C9

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

ALDH4, ALDH7, ALDH9, ALDH9A1, E3, TMABADH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67372 targets ALDH9A1 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24493

Product name: Recombinant human ALDH9A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 285-412 aa of BC151140

Sequence: LIIFSDCDMNNAVKGALMANFLTQGQVCCNGTRVFVQKEILDKFTEEVVKQTQRIKIGDPLLEDTRMGPLINRPHLERVLGFVKVAKEQGAKVLCGGDIYVPEDPKLKDGYYMRPCVLTNCRDDMTCV

相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 9 family, member A1
Calculated molecular weight 518 aa, 56 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC151140
Gene Symbol ALDH9A1
Gene ID (NCBI) 223
RRIDAB_2919459
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP49189
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aldehyde dehydrogenase 9 family member A1(ALDH9A1), belongs to the aldehyde dehydrogenase family of proteins. ALDH9A1 has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. ALDH9A1 catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid. ALDH9A1 has 3 isoforms with the molecular mass of 46-56 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ALDH9A1 antibody CL488-67372Download protocol
Standard Protocols
Click here to view our Standard Protocols