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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDH2 Recombinant antibody (86429-5-RR)

    WB analysis using 86429-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86429-5-RR (ALDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using ALDH2 Recombinant antibody (86429-5-RR)

    IF Staining of C2C12 using 86429-5-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using ALDH2 antibody (86429-5-RR, Clone: 251287E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ALDH2 Recombinant antibody (86429-5-RR)

    IF Staining of A549 using 86429-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ALDH2 antibody (86429-5-RR, Clone: 251287E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86429-5-RR

    Affinity and Kinetic Characterization of 86429-5-RR

    Biolayer interferometry (BLl) kinetic assays of 86429-5-RR against Human ALDH2 were performed. The affinity constant is below 1 pM.

ALDH2 Recombinant monoclonal antibody

ALDH2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86429-5-RR
Clone No.251287E9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ALDH 2, ALDH class 2, ALDH E2, ALDHE2, ALDH-E2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, SK-BR-3 cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inC2C12 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86429-5-RR targets ALDH2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALDH2 fusion protein Ag7452 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 2 family (mitochondrial)
Calculated molecular weight 56 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC002967
Gene Symbol ALDH2
Gene ID (NCBI) 217
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP05091
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

Protocols

Product Specific Protocols
WB protocol for ALDH2 antibody 86429-5-RRDownload protocol
IF protocol for ALDH2 antibody 86429-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols