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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDH2 Recombinant antibody (86429-5-RR)

    WB analysis using 86429-5-RR (same clone as 86429-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86429-5-RR (ALDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86429-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using ALDH2 Recombinant antibody (86429-5-RR)

    IF Staining of C2C12 using 86429-5-RR (same clone as 86429-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using ALDH2 antibody (86429-5-RR, Clone: 251287E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86429-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ALDH2 Recombinant antibody (86429-5-RR)

    IF Staining of A549 using 86429-5-RR (same clone as 86429-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ALDH2 antibody (86429-5-RR, Clone: 251287E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86429-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86429-5-RR

    Affinity and Kinetic Characterization of 86429-5-RR

    Biolayer interferometry (BLl) kinetic assays of 86429-5-RR against Human ALDH2 were performed. The affinity constant is below 1 pM.

ALDH2 Recombinant monoclonal antibody, PBS Only

ALDH2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86429-5-PBS
Clone No.251287E9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ALDH 2, ALDH class 2, ALDH E2, ALDHE2, ALDH-E2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86429-5-PBS targets ALDH2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALDH2 fusion protein Ag7452 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 2 family (mitochondrial)
Calculated molecular weight 56 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC002967
Gene Symbol ALDH2
Gene ID (NCBI) 217
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP05091
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.