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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using AKR7A2 Monoclonal antibody (66677-1-Ig)

    WB analysis of Jurkat using 66677-1-Ig (same clone as 66677-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66677-1-Ig (AKR7A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AKR7A2 Monoclonal antibody (66677-1-Ig)

    WB analysis of HEK-293 using 66677-1-Ig (same clone as 66677-1-PBS)

    WB result of AKR7A2 antibody (66677-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR7A2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AKR7A2 Monoclonal antibody (66677-1-Ig)

    WB analysis of HEK-293 using 66677-1-Ig (same clone as 66677-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66677-1-Ig (AKR7A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AKR7A2 Monoclonal antibody (66677-1-Ig)

    IHC staining of human liver cancer using 66677-1-Ig (same clone as 66677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66677-1-Ig (AKR7A2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AKR7A2 Monoclonal antibody (66677-1-Ig)

    IHC staining of human liver cancer using 66677-1-Ig (same clone as 66677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66677-1-Ig (AKR7A2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AKR7A2 Monoclonal antibody (66677-1-Ig)

    IF Staining of HepG2 using 66677-1-Ig (same clone as 66677-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AKR7A2 antibody (66677-1-Ig, Clone: 2E1E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AKR7A2 Monoclonal antibody, PBS Only

AKR7A2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66677-1-PBS
Clone No.2E1E8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66677-1-PBS targets AKR7A2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26105

Product name: Recombinant human AKR7A2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 60-359 aa of BC007352

Sequence: RAFLERGHTELDTAFMYSDGQSETILGGLGLGLGGGDCRVKIATKANPWDGKSLKPDSVRSQLETSLKRLQCPQVDLFYLHAPDHGTPVEETLHACQRLHQEGKFVELGLSNYASWEVAEICTLCKSNGWILPTVYQGMYNATTRQVETELFPCLRHFGLRFYAYNPLAGGLLTGKYKYEDKDGKQPVGRFFGNSWAETYRNRFWKEHHFEAIALVEKALQAAYGASAPSVTSAALRWMYHHSQLQGAHGDAVILGMSSLEQLEQNLAATEEGPLEPAVVDAFNQAWHLVAHECPNYFR

相同性解析による交差性が予測される生物種
Full Name aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
Calculated molecular weight 40 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC007352
Gene Symbol AKR7A2
Gene ID (NCBI) 8574
RRIDAB_2882031
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO43488
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AKR7A2(Aflatoxin B1 aldehyde reductase member 2) is also named as AFAR, AFAR1, AKR7 and belongs to the aldo/keto reductase family. AKR7A2 enzyme is catalytically active toward aldehydes arising from lipid peroxidation, suggesting a potential role against the consequences of oxidative stress, and representing an important detoxication route in mammalian cells(PMID:22001351).

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