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  • Featured Product
  • KD/KO Validated

AKR7A2 Monoclonal antibody

AKR7A2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E1E8

Cat no : 66677-1-Ig

Synonyms

AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 66677-1-Ig

    WB analysis of HEK-293 using 66677-1-Ig

    WB result of AKR7A2 antibody (66677-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR7A2 transfected HEK-293 cells.

  • WB analysis of Jurkat using 66677-1-Ig

    WB analysis of Jurkat using 66677-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66677-1-Ig (AKR7A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66677-1-Ig

    WB analysis of HEK-293 using 66677-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66677-1-Ig (AKR7A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66677-1-Ig

    IHC staining of human liver cancer using 66677-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66677-1-Ig (AKR7A2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66677-1-Ig

    IHC staining of human liver cancer using 66677-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66677-1-Ig (AKR7A2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 66677-1-Ig

    IF Staining of HepG2 using 66677-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AKR7A2 antibody (66677-1-Ig, Clone: 2E1E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66677-1-Ig targets AKR7A2 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AKR7A2 fusion protein Ag26105 相同性解析による交差性が予測される生物種
Full Name aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
Calculated molecular weight 40 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC007352
Gene symbol AKR7A2
Gene ID (NCBI) 8574
RRIDAB_2882031
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKR7A2(Aflatoxin B1 aldehyde reductase member 2) is also named as AFAR, AFAR1, AKR7 and belongs to the aldo/keto reductase family. AKR7A2 enzyme is catalytically active toward aldehydes arising from lipid peroxidation, suggesting a potential role against the consequences of oxidative stress, and representing an important detoxication route in mammalian cells(PMID:22001351).

Protocols

Product Specific Protocols
WB protocol for AKR7A2 antibody 66677-1-IgDownload protocol
IHC protocol for AKR7A2 antibody 66677-1-IgDownload protocol
IF protocol for AKR7A2 antibody 66677-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols