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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using AKAP11-Specific Polyclonal antibody (30552-1-AP)

    WB analysis of MCF-7 using 30552-1-AP (same clone as 30552-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 30552-1-AP (AKAP11-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30552-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using AKAP11-Specific Polyclonal antibody (30552-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30552-1-AP (same clone as 30552-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30552-1-AP (AKAP11-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30552-1-PBS in a different storage buffer formulation.

AKAP11-Specific Polyclonal antibody, PBS Only

AKAP11-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30552-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

AKAP11, A-kinase anchor protein 11, AKAP220, AKAP-11, AKAP 220

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30552-1-PBS targets AKAP11-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31539

Product name: Recombinant human AKAP11-Specific protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1642-1808 aa of NM_016248

Sequence: VNLDKKAVLAEKIVAEAIEKAERELSSTSLAADSGIGQEGASFAESLATETMTAAVTNVGHAVSSSKEIEDFQSTESVSSQQMNLSIGDDSTGSWSNLSFEDEHQDESSSFHHLSESNGNSSSWSSLGLEGDLYEDNLSFPTSDSDGPDDKDEEHEDEVEGLGQDGK

相同性解析による交差性が予測される生物種
Full Name A kinase (PRKA) anchor protein 11
Calculated molecular weight 211 kDa
Observed molecular weight250 kDa
GenBank accession numberNM_016248
Gene Symbol AKAP11
Gene ID (NCBI) 11215
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKA4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AKAP11, also named as AKAP220, KIAA0629 and PRKA11, belongs to the AKAP110 family. AKAP11 binds to type II regulatory subunits of protein kinase A and anchors/targets them. The antibody is specific to AKAP11.

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