Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30552-1-AP targets AKAP11-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31539 Product name: Recombinant human AKAP11-Specific protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1642-1808 aa of NM_016248 Sequence: VNLDKKAVLAEKIVAEAIEKAERELSSTSLAADSGIGQEGASFAESLATETMTAAVTNVGHAVSSSKEIEDFQSTESVSSQQMNLSIGDDSTGSWSNLSFEDEHQDESSSFHHLSESNGNSSSWSSLGLEGDLYEDNLSFPTSDSDGPDDKDEEHEDEVEGLGQDGK 相同性解析による交差性が予測される生物種 |
| Full Name | A kinase (PRKA) anchor protein 11 |
| Calculated molecular weight | 211 kDa |
| Observed molecular weight | 250 kDa |
| GenBank accession number | NM_016248 |
| Gene Symbol | AKAP11 |
| Gene ID (NCBI) | 11215 |
| RRID | AB_3086360 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKA4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AKAP11, also named as AKAP220, KIAA0629 and PRKA11, belongs to the AKAP110 family. AKAP11 binds to type II regulatory subunits of protein kinase A and anchors/targets them. The antibody is specific to AKAP11.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AKAP11-Specific antibody 30552-1-AP | Download protocol |
| WB protocol for AKAP11-Specific antibody 30552-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |