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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGMAT Polyclonal antibody (21899-1-AP)

    WB analysis using 21899-1-AP (same clone as 21899-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21899-1-AP (AGMAT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21899-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AGMAT Polyclonal antibody (21899-1-AP)

    WB analysis using 21899-1-AP (same clone as 21899-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21899-1-AP (AGMAT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21899-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using AGMAT Polyclonal antibody (21899-1-AP)

    IHC staining of human stomach cancer using 21899-1-AP (same clone as 21899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21899-1-AP (AGMAT antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21899-1-PBS in a different storage buffer formulation.

AGMAT Polyclonal antibody, PBS Only

AGMAT Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21899-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Agmatinase, Arginase, mitochondrial, Agmatine ureohydrolase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21899-1-PBS targets AGMAT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13609

Product name: Recombinant human AGMAT protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-300 aa of BC005090

Sequence: MLRLLASGCARGPGPGVGARPAAGLFHPGRRQSRQASDAPRNQPPSPEFVARPVGVCSMMRLPVQTSPEGLDAAFIGVPLDTGTSNRPGARFGPRRIREESVMLRTVNPSTGALPFQSLMVADLGDVNVNLYNLQDSCRRIQEAYEKIVAAGCIPLTLGGDHTITYPILQAMAKKHGPVGLLHVDAHTDTTDKALGEKLYHGAPFRRCVDEGLLDCKRVVQIGIRGSSTTLDPYRYNRSQGFRVVLAEDCWMKSLVPLMGEVRQQMGGKPIYISFDIDALDPAYAPGTGTPEIAGLTPSQ

相同性解析による交差性が予測される生物種
Full Name agmatine ureohydrolase (agmatinase)
Calculated molecular weight 38 kDa
Observed molecular weight38 kDa
GenBank accession numberBC005090
Gene Symbol AGMAT
Gene ID (NCBI) 79814
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSE5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AGMAT is a metallohydrolase and belongs to the arginase family. AGMAT plays an important role in amino acid metabolism and can hydrolyze agmatine into urea and putrescine. AGMAT has been shown to play an important role in the development of tumors, including colorectal cancer, lung adenocarcinoma, and pancreatic adenocarcinoma(PMID: 36680755; PMID: 31699997; PMID: 35837051 ).

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