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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGMAT Polyclonal antibody (21899-1-AP)

    WB analysis using 21899-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21899-1-AP (AGMAT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AGMAT Polyclonal antibody (21899-1-AP)

    WB analysis using 21899-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21899-1-AP (AGMAT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using AGMAT Polyclonal antibody (21899-1-AP)

    IHC staining of human stomach cancer using 21899-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21899-1-AP (AGMAT antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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AGMAT Polyclonal antibody

AGMAT Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21899-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Agmatinase, Arginase, mitochondrial, Agmatine ureohydrolase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21899-1-AP targets AGMAT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGMAT fusion protein Ag13609 相同性解析による交差性が予測される生物種
Full Name agmatine ureohydrolase (agmatinase)
Calculated molecular weight 38 kDa
Observed molecular weight38 kDa
GenBank accession numberBC005090
Gene Symbol AGMAT
Gene ID (NCBI) 79814
RRIDAB_3085677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AGMAT is a metallohydrolase and belongs to the arginase family. AGMAT plays an important role in amino acid metabolism and can hydrolyze agmatine into urea and putrescine. AGMAT has been shown to play an important role in the development of tumors, including colorectal cancer, lung adenocarcinoma, and pancreatic adenocarcinoma(PMID: 36680755; PMID: 31699997; PMID: 35837051 ).

Protocols

Product Specific Protocols
WB protocol for AGMAT antibody 21899-1-APDownload protocol
IHC protocol for AGMAT antibody 21899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols