Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, mouse skeletal muscle tissue, Jurkat cells, K-562 cells, rat skeletal muscle tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86187-1-RR targets AGL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | AGL fusion protein Ag9962 相同性解析による交差性が予測される生物種 |
Full Name | amylo-1, 6-glucosidase, 4-alpha-glucanotransferase |
Calculated molecular weight | 1532 aa, 175 kDa |
Observed molecular weight | 165 kDa |
GenBank accession number | BC078663 |
Gene Symbol | AGL |
Gene ID (NCBI) | 178 |
Conjugate | Unconjugated |
Form | |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P35573 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Amylo-alpha-1-6-glucosidase-4-alpha-glucanotransferase (AGL) (also known as glycogen debranching enzyme) is a critical bifunctional enzyme involved in glycogen metabolism.
Protocols
Product Specific Protocols | |
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WB protocol for AGL antibody 86187-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |