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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGL Recombinant monoclonal antibody (86187-1-RR)

    WB analysis using 86187-1-RR (same clone as 86187-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86187-1-RR (AGL antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86187-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AGL Recombinant monoclonal antibody (86187-1-RR)

    WB analysis using 86187-1-RR (same clone as 86187-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86187-1-RR (AGL antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86187-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86187-1-RR

    Affinity and Kinetic Characterization of 86187-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86187-1-RR against Human AGL were performed. The affinity constant is below 1 pM.

AGL Recombinant monoclonal antibody, PBS Only

AGL Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86187-1-PBS
Clone No.250767A6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

4-alpha-glucanotransferase, Amylo-1,6-glucosidase, Amylo-alpha-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, Glycogen debrancher

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86187-1-PBS targets AGL in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9962

Product name: Recombinant human AGL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1182-1533 aa of BC078663

Sequence: MYPTDDSAPLPAGTLDQPLFEVIQEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAIKVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLERSPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL

相同性解析による交差性が予測される生物種
Full Name amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
Calculated molecular weight 1532 aa, 175 kDa
Observed molecular weight 165 kDa
GenBank accession numberBC078663
Gene Symbol AGL
Gene ID (NCBI) 178
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP35573
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Amylo-alpha-1-6-glucosidase-4-alpha-glucanotransferase (AGL) (also known as glycogen debranching enzyme) is a critical bifunctional enzyme involved in glycogen metabolism.

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