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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated AGER/RAGE Monoclonal (CL488-66833)

    WB analysis using CL488-66833

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66833 (AGER antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of rat lung tissue using CoraLite® Plus 488-conjugated AGER Monoclonal anti (CL488-66833)

    IF Staining of rat lung using CL488-66833

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using CoraLite® Plus 488 AGER antibody (CL488-66833, Clone: 3D4G11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 488-conjugated AGER/RAGE Monoclonal antibody

AGER/RAGE Monoclonal Antibody for WB, IF-P
Cat No. CL488-66833
Clone No.3D4G11

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IF-P

Synonyms

RAGE, AGER, Advanced glycosylation end product-specific receptor, 3D4G11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig lung tissue
Positive IF-P detected inrat lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66833 targets AGER/RAGE in WB, IF-P applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AGER/RAGE fusion protein Ag9657 相同性解析による交差性が予測される生物種
Full Name advanced glycosylation end product-specific receptor
Calculated molecular weight 404 aa, 43 kDa
Observed molecular weight 43, 50 kDa
GenBank accession numberBC020669
Gene Symbol AGER
Gene ID (NCBI) 177
RRIDAB_3672924
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15109
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 AGER/RAGE antibody CL488-66833Download protocol
IF protocol for CL Plus 488 AGER/RAGE antibody CL488-66833Download protocol
Standard Protocols
Click here to view our Standard Protocols