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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis using 14631-1-AP (same clone as 14631-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

    WB result of AFG3L2 antibody (14631-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFG3L2 transfected HeLa cells. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis using 14631-1-AP (same clone as 14631-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis of mouse skeletal muscle using 14631-1-AP (same clone as 14631-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis of mouse kidney using 14631-1-AP (same clone as 14631-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using AFG3L2 Polyclonal antibody (14631-1-AP)

    IP experiment of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

    IP result of anti-AFG3L2 (IP:14631-1-AP, 4ug; Detection:14631-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AFG3L2 Polyclonal antibody (14631-1-AP)

    IF Staining of HepG2 using 14631-1-AP (same clone as 14631-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AFG3L2 Polyclonal antibody (14631-1-AP)

    IF Staining of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AFG3L2 Polyclonal antibody, PBS Only

AFG3L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14631-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14631-1-PBS targets AFG3L2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6209

Product name: Recombinant human AFG3L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 449-797 aa of BC065016

Sequence: ILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSTLEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN

相同性解析による交差性が予測される生物種
Full Name AFG3 ATPase family gene 3-like 2 (yeast)
Calculated molecular weight 88 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC065016
Gene Symbol AFG3L2
Gene ID (NCBI) 10939
RRIDAB_2242420
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4W6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).

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