Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 14631-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 14631-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14631-1-AP
WB result of AFG3L2 antibody (14631-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFG3L2 transfected HeLa cells.
WB analysis of mouse skeletal muscle using 14631-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 14631-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14631-1-AP
IP result of anti-AFG3L2 (IP:14631-1-AP, 4ug; Detection:14631-1-AP 1:5000) with HeLa cells lysate 1560 ug.
IHC staining of human kidney using 14631-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 14631-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human kidney using 14631-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 14631-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 14631-1-AP
Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 14631-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

AFG3L2 Polyclonal antibody

AFG3L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14631-1-AP

Publications(42)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

AFG3 like protein 2, AFG3L 2, AFG3-like protein 2, EC:3.4.24.-, Mitochondrial inner membrane m-AAA protease component AFG3L2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (42)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse skeletal muscle tissue, mouse kidney tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 37 publications below
IF	See 1 publications below
IP	See 3 publications below
CoIP	See 1 publications below

Product Information

14631-1-AP targets AFG3L2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6209 Product name: Recombinant human AFG3L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 449-797 aa of BC065016 Sequence: ILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSTLEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN 相同性解析による交差性が予測される生物種
Full Name	AFG3 ATPase family gene 3-like 2 (yeast)
Calculated molecular weight	88 kDa
Observed molecular weight	80-90 kDa
GenBank accession number	BC065016
Gene Symbol	AFG3L2
Gene ID (NCBI)	10939
RRID	AB_2242420
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y4W6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).

Protocols

Product Specific Protocols
IF protocol for AFG3L2 antibody 14631-1-AP	Download protocol
IHC protocol for AFG3L2 antibody 14631-1-AP	Download protocol
IP protocol for AFG3L2 antibody 14631-1-AP	Download protocol
WB protocol for AFG3L2 antibody 14631-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol A transport-independent role for SLC25A12 in mitochondrial stress signalling Authors - Liu Jiang View Article
human	IF	Cancer Res Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition Authors - Liang Fang View Article
mouse	WB	Autophagy Mitochondrial dynamics and quality control regulate proteostasis in neuronal ischemia-reperfusion Authors - Garrett M Fogo View Article
human	WB	Nat Commun FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes Authors - Jiamin Qiu View Article
human	WB	Nat Commun TANGO2 binds crystallin alpha B and its loss causes desminopathy Authors - Maike Stentenbach View Article
human	WB	Mol Cell The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism Authors - Wang Jiahui KD Validated View Article

Validation Data Gallery

WB analysis using 14631-1-AP

WB analysis using 14631-1-AP

WB analysis of HeLa using 14631-1-AP

WB analysis of mouse skeletal muscle using 14631-1-AP

WB analysis of mouse kidney using 14631-1-AP

IP experiment of HeLa using 14631-1-AP

IHC staining of human kidney using 14631-1-AP

IHC staining of human kidney using 14631-1-AP

IHC staining of human kidney using 14631-1-AP

IHC staining of human kidney using 14631-1-AP

IF Staining of HepG2 using 14631-1-AP

IF Staining of HeLa using 14631-1-AP

AFG3L2 Polyclonal antibody

AFG3L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A transport-independent role for SLC25A12 in mitochondrial stress signalling

Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition

Mitochondrial dynamics and quality control regulate proteostasis in neuronal ischemia-reperfusion

FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes

TANGO2 binds crystallin alpha B and its loss causes desminopathy

The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism

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