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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ADRP/Perilipin 2 Polyclonal antibody

ADRP/Perilipin 2 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15294

Synonyms

ADFP, Adipophilin, ADRP, ADRP/Perilipin 2, Perilipin 2, PLIN2

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-15294

    IF Staining of HeLa using CL488-15294

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite® Plus 488 ADRP/Perilipin 2 antibody (CL488-15294) at dilution of 1:400, CL594-Phalloidine (red).

  • IF Staining of HeLa using CL488-15294

    IF Staining of HeLa using CL488-15294

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite® Plus 488 ADRP/Perilipin 2 antibody (CL488-15294) at dilution of 1:400.

Tested Applications

Positive IF detected inoleic acid treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ADRP Ready-To-Use IHC Kit. ADRP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15294-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL488-15294 targets ADRP/Perilipin 2 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRP/Perilipin 2 fusion protein Ag7539 相同性解析による交差性が予測される生物種
Full Name adipose differentiation-related protein
Calculated molecular weight 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC005127
Gene symbol ADFP
Gene ID (NCBI) 123
RRIDAB_2919116
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ADRP/Perilipin 2 antibody CL488-15294Download protocol
Standard Protocols
Click here to view our Standard Protocols