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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ADCK5 Polyclonal antibody (17579-1-AP)

    WB analysis of mouse brain using 17579-1-AP (same clone as 17579-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17579-1-AP (ADCK5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17579-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ADCK5 Polyclonal antibody (17579-1-AP)

    WB analysis of mouse brain using 17579-1-AP (same clone as 17579-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17579-1-AP (ADCK5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17579-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ADCK5 Polyclonal antibody (17579-1-AP)

    IHC staining of human ovary cancer using 17579-1-AP (same clone as 17579-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17579-1-AP (ADCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17579-1-PBS in a different storage buffer formulation.

ADCK5 Polyclonal antibody, PBS Only

ADCK5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17579-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:2.7.11.-, Uncharacterized aarF domain-containing protein kinase 5

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17579-1-PBS targets ADCK5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11094

Product name: Recombinant human ADCK5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-169 aa of BC031570

Sequence: MRAHAAALGVQDYLLFAEMLMQRPVRLGQLWGSHLLSREEAAYMVDMARERFEAVMAVLRELPRPMLLVLRNINTVRAINVALGAPVDRYFLMAKRAVRGWSRLAGATYRGVYGTSLLRHAKVVWEMLKFEVALRLETLAMRLTALLARALVHLSLVPPAEELYQYLET

相同性解析による交差性が予測される生物種
Full Name aarF domain containing kinase 5
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC031570
Gene Symbol ADCK5
Gene ID (NCBI) 203054
RRIDAB_2242313
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3MIX3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ADCK5 (AarF Domain Containing Kinase 5) is a protein kinase belonging to the ADCK protein family. Named for its AarF domain, this family plays important roles in cellular energy metabolism and mitochondrial function regulation. ADCK5 is primarily expressed in human tissues and participates in the biosynthesis of Coenzyme Q (CoQ). CoQ is a critical component of the mitochondrial electron transport chain, essential for cellular energy production. ADCK5 contains ubiquitination modification sites, and the experimentally detected molecular weight may range between 65-75 kDa.

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