Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17579-1-AP targets ADCK5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11094 Product name: Recombinant human ADCK5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-169 aa of BC031570 Sequence: MRAHAAALGVQDYLLFAEMLMQRPVRLGQLWGSHLLSREEAAYMVDMARERFEAVMAVLRELPRPMLLVLRNINTVRAINVALGAPVDRYFLMAKRAVRGWSRLAGATYRGVYGTSLLRHAKVVWEMLKFEVALRLETLAMRLTALLARALVHLSLVPPAEELYQYLET 相同性解析による交差性が予測される生物種 |
| Full Name | aarF domain containing kinase 5 |
| Calculated molecular weight | 66 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC031570 |
| Gene Symbol | ADCK5 |
| Gene ID (NCBI) | 203054 |
| RRID | AB_2242313 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3MIX3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADCK5 (AarF Domain Containing Kinase 5) is a protein kinase belonging to the ADCK protein family. Named for its AarF domain, this family plays important roles in cellular energy metabolism and mitochondrial function regulation. ADCK5 is primarily expressed in human tissues and participates in the biosynthesis of Coenzyme Q (CoQ). CoQ is a critical component of the mitochondrial electron transport chain, essential for cellular energy production. ADCK5 contains ubiquitination modification sites, and the experimentally detected molecular weight may range between 65-75 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ADCK5 antibody 17579-1-AP | Download protocol |
| WB protocol for ADCK5 antibody 17579-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |