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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSM2A Polyclonal antibody (22862-1-AP)

    WB analysis using 22862-1-AP (same clone as 22862-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22862-1-AP (ACSM2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using ACSM2A Polyclonal antibody (22862-1-AP)

    WB analysis of human brain using 22862-1-AP (same clone as 22862-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 22862-1-AP (ACSM2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM2A Polyclonal antibody (22862-1-AP)

    IHC staining of human liver cancer using 22862-1-AP (same clone as 22862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22862-1-AP (ACSM2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ACSM2A Polyclonal antibody (22862-1-AP)

    IHC staining of human liver cancer using 22862-1-AP (same clone as 22862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22862-1-AP (ACSM2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACSM2A Polyclonal antibody (22862-1-AP)

    IF Staining of HepG2 using 22862-1-AP (same clone as 22862-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 22862-1-AP (ACSM2A antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22862-1-PBS in a different storage buffer formulation.

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ACSM2A Polyclonal antibody, PBS Only

ACSM2A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22862-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22862-1-PBS targets ACSM2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSM2A fusion protein Ag18856 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase medium-chain family member 2A
Calculated molecular weight 577 aa, 64 kDa
Observed molecular weight 60-64 kDa
GenBank accession numberBC125176
Gene Symbol ACSM2A
Gene ID (NCBI) 123876
RRIDAB_2879181
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08AH3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ACSM2A(Acyl-coenzyme A synthetase ACSM2A, mitochondrial) is also named as ACSM2, MACS2 and belongs to the ATP-dependent AMP-binding enzyme family. It acts as a medium-chain fatty acid : CoA ligase with the caculated molecular weight of 64 kDa and acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids.