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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACSM2A Polyclonal antibody

ACSM2A Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22862-1-AP

Synonyms

A 923A4.1, ACSM2, ACSM2A, Butyrate CoA ligase 2A, MACS2

Validation Data Gallery

Applications:
  • WB analysis using 22862-1-AP

    WB analysis using 22862-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22862-1-AP (ACSM2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 22862-1-AP

    WB analysis of human brain using 22862-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22862-1-AP (ACSM2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 22862-1-AP

    IHC staining of human liver cancer using 22862-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22862-1-AP (ACSM2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 22862-1-AP

    IHC staining of human liver cancer using 22862-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22862-1-AP (ACSM2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 22862-1-AP

    IF Staining of HepG2 using 22862-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 22862-1-AP (ACSM2A antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, human brain tissue, rat liver tissue, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 22862-1-AP is ACSM2A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSM2A fusion protein Ag18856 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase medium-chain family member 2A
Calculated molecular weight 577 aa, 64 kDa
Observed molecular weight 60-64 kDa
GenBank accession numberBC125176
Gene symbol ACSM2A
Gene ID (NCBI) 123876
RRIDAB_2879181
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACSM2A(Acyl-coenzyme A synthetase ACSM2A, mitochondrial) is also named as ACSM2, MACS2 and belongs to the ATP-dependent AMP-binding enzyme family. It acts as a medium-chain fatty acid : CoA ligase with the caculated molecular weight of 64 kDa and acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids.

Protocols

Product Specific Protocols
WB protocol for ACSM2A antibody 22862-1-APDownload protocol
IHC protocol for ACSM2A antibody 22862-1-APDownload protocol
IF protocol for ACSM2A antibody 22862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Front Physiol

Proteomics analyses of acute kidney injury biomarkers in a rat exertional heat stroke model

Authors - Fu-Li Wen
View Article