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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACSL5 Polyclonal antibody (15708-1-AP)

    WB analysis using 15708-1-AP (same clone as 15708-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15708-1-AP (ACSL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ACSL5 Polyclonal antibody (15708-1-AP)

    IP experiment of mouse brain using 15708-1-AP (same clone as 15708-1-PBS)

    IP result of anti-ACSL5 (IP:15708-1-AP, 4ug; Detection:15708-1-AP 1:600) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ACSL5 Polyclonal antibody (15708-1-AP)

    IHC staining of human colon cancer using 15708-1-AP (same clone as 15708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15708-1-AP (ACSL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ACSL5 Polyclonal antibody (15708-1-AP)

    IHC staining of human colon cancer using 15708-1-AP (same clone as 15708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15708-1-AP (ACSL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

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ACSL5 Polyclonal antibody, PBS Only

ACSL5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15708-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

LACS 5, FACL5, EC:6.2.1.3, EC:6.2.1.15, Arachidonate--CoA ligase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15708-1-PBS targets ACSL5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSL5 fusion protein Ag8258 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase long-chain family member 5
Calculated molecular weight 76 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC007985
Gene Symbol ACSL5
Gene ID (NCBI) 51703
RRIDAB_2257881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULC5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Acyl-CoA synthetase 5, a member of the ACSL gene family that catalyzes the activation of long-chain fatty acids for lipid biosynthesis, is the only ACSL isoform that is both, located on mitochondria and functionally involved in enterocyte apoptosis.ACSL5 could play a role in promoting fatty acid-induced lipoapoptosis in hepatocytes as important mechanism in fatty liver-related disorders(PMID:20470896). ACSL5 is most abundant in liver, brown adipose tissue, and intestine and is located on both the mitochondrial membrane and endoplasmic reticulum(PMID:20798351). It has 3 isoforms produced by alternative splicing.