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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using ACOT4 Polyclonal antibody (17316-1-AP)

    WB analysis of L02 using 17316-1-AP (same clone as 17316-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ACOT4 Polyclonal antibody (17316-1-AP)

    WB analysis using 17316-1-AP (same clone as 17316-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ACOT4 Polyclonal antibody (17316-1-AP)

    IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ACOT4 Polyclonal antibody (17316-1-AP)

    IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ACOT4 Polyclonal antibody (17316-1-AP)

    IF Staining of A549 using 17316-1-AP (same clone as 17316-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACOT4 antibody (17316-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

ACOT4 Polyclonal antibody, PBS Only

ACOT4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17316-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17316-1-PBS targets ACOT4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11286

Product name: Recombinant human ACOT4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-225 aa of BC031799

Sequence: NNMDNISLEYFEEAVCYMLQHPQVKGPGIGLLGISLGADICLSMASFLKNVSATVSINGSGISGNTAINYKHSSIPPLGYDLRRIKVAFSGLVDIVDIRNALVGGYKNPSMIPIEKAQGPILLIVGQDDHNWRSELYAQTVSERLQAHGKEKPQIICYPGTGHYIEPPYFPLCPASLHRLLNKHVIWGGEPRAHSKAQEDAWKQILAFFCKHLGGTQKTAVPKL

相同性解析による交差性が予測される生物種
Full Name acyl-CoA thioesterase 4
Calculated molecular weight 421 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC031799
Gene Symbol ACOT4
Gene ID (NCBI) 122970
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9L9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACOT4, also known as PET2B and PTEIB, is an acyl-CoA thioesterases which catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), regulating their respective intracellular levels. ACOT4 has the strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle(PMID: 16940157).

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