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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ACOT4 Polyclonal antibody

ACOT4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17316-1-AP

Synonyms

ACOT4, acyl CoA thioesterase 4, Acyl coenzyme A thioesterase 4, PTE 2b, PTE Ib, PTE1B, PTE2B, PTEIB

Validation Data Gallery

Applications:
  • WB analysis of L02 using 17316-1-AP

    WB analysis of L02 using 17316-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 17316-1-AP

    IHC staining of mouse kidney using 17316-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 17316-1-AP

    IHC staining of mouse kidney using 17316-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 17316-1-AP

    IF Staining of A549 using 17316-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACOT4 antibody (17316-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

17316-1-AP targets ACOT4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOT4 fusion protein Ag11286 相同性解析による交差性が予測される生物種
Full Name acyl-CoA thioesterase 4
Calculated molecular weight 421 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC031799
Gene symbol ACOT4
Gene ID (NCBI) 122970
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACOT4, also known as PET2B and PTEIB, is an acyl-CoA thioesterases which catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), regulating their respective intracellular levels. ACOT4 has the strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle(PMID: 16940157).

Protocols

Product Specific Protocols
WB protocol for ACOT4 antibody 17316-1-APDownload protocol
IHC protocol for ACOT4 antibody 17316-1-APDownload protocol
IF protocol for ACOT4 antibody 17316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols