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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using ACE Polyclonal antibody (24743-1-AP)

    WB analysis of mouse heart using 24743-1-AP (same clone as 24743-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 24743-1-AP (ACE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat lung tissue using ACE Polyclonal antibody (24743-1-AP)

    WB analysis of rat lung using 24743-1-AP (same clone as 24743-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 24743-1-AP (ACE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using ACE Polyclonal antibody (24743-1-AP)

    IHC staining of rat lung using 24743-1-AP (same clone as 24743-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using ACE Polyclonal antibody (24743-1-AP)

    IHC staining of human lung using 24743-1-AP (same clone as 24743-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using ACE Polyclonal antibody (24743-1-AP)

    IHC staining of human lung using 24743-1-AP (same clone as 24743-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ACE Polyclonal antibody (24743-1-AP)

    IHC staining of mouse lung using 24743-1-AP (same clone as 24743-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ACE Polyclonal antibody (24743-1-AP)

    IHC staining of mouse lung using 24743-1-AP (same clone as 24743-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24743-1-PBS in a different storage buffer formulation.

ACE Polyclonal antibody, PBS Only

ACE Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24743-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

DCP1, DCP, Angiotensin-converting enzyme, soluble form, Angiotensin-converting enzyme, Angiotensin converting enzyme

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24743-1-PBS targets ACE in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20654

Product name: Recombinant human ACE protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 390-739 aa of BC036375

Sequence: FYNGKDFRIKQCTTVNLEDLVVAHHEMGHIQYFMQYKDLPVALREGANPGFHEAIGDVLALSVSTPKHLHSLNLLSSEGGSDEHDINFLMKMALDKIAFIPFSYLVDQWRWRVFDGSITKENYNQEWWSLRLKYQGLCPPVPRTQGDFDPGAKFHIPSSVPYIRYFVSFIIQFQFHEALCQAAGHTGPLHKCDIYQSKEAGQRLATAMKLGFSRPWPEAMQLITGQPNMSASAMLSYFKPLLDWLRTENELHGEKLGWPQYNWTPNSDDFYNETETKIFLQFYDQTGIWDHGAPHLLPPSQARGTREAPVYMSKRAASSGPPGSPKLPPAAQGHGEVPVHDLLWHPGPPV

相同性解析による交差性が予測される生物種
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Calculated molecular weight 150 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC036375
Gene Symbol ACE
Gene ID (NCBI) 1636
RRIDAB_2879701
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP12821
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).

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