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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using ACE Polyclonal antibody (24743-1-AP)

    WB analysis of mouse heart using 24743-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 24743-1-AP (ACE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat lung tissue using ACE Polyclonal antibody (24743-1-AP)

    WB analysis of rat lung using 24743-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 24743-1-AP (ACE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat lung tissue using ACE Polyclonal antibody (24743-1-AP)

    IHC staining of rat lung using 24743-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using ACE Polyclonal antibody (24743-1-AP)

    IHC staining of human lung using 24743-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using ACE Polyclonal antibody (24743-1-AP)

    IHC staining of human lung using 24743-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ACE Polyclonal antibody (24743-1-AP)

    IHC staining of mouse lung using 24743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ACE Polyclonal antibody (24743-1-AP)

    IHC staining of mouse lung using 24743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using ACE Polyclonal antibody (24743-1-AP)

    IF Staining of mouse heart using 24743-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ACE antibody (24743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using ACE Polyclonal antibody (24743-1-AP)

    IF Staining of mouse heart using 24743-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ACE antibody (24743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACE Polyclonal antibody

ACE Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 24743-1-AP
Publications(53)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, CoIP, ELISA

Synonyms

ACE1, Angiotensin converting enzyme, Angiotensin-converting enzyme, Angiotensin-converting enzyme, soluble form, DCP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inrat lung tissue, human lung tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24743-1-AP targets ACE in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20654

Product name: Recombinant human ACE protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 390-739 aa of BC036375

Sequence: FYNGKDFRIKQCTTVNLEDLVVAHHEMGHIQYFMQYKDLPVALREGANPGFHEAIGDVLALSVSTPKHLHSLNLLSSEGGSDEHDINFLMKMALDKIAFIPFSYLVDQWRWRVFDGSITKENYNQEWWSLRLKYQGLCPPVPRTQGDFDPGAKFHIPSSVPYIRYFVSFIIQFQFHEALCQAAGHTGPLHKCDIYQSKEAGQRLATAMKLGFSRPWPEAMQLITGQPNMSASAMLSYFKPLLDWLRTENELHGEKLGWPQYNWTPNSDDFYNETETKIFLQFYDQTGIWDHGAPHLLPPSQARGTREAPVYMSKRAASSGPPGSPKLPPAAQGHGEVPVHDLLWHPGPPV

相同性解析による交差性が予測される生物種
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Calculated molecular weight 150 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC036375
Gene Symbol ACE
Gene ID (NCBI) 1636
RRIDAB_2879701
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP12821
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).

Protocols

Product Specific Protocols
IF protocol for ACE antibody 24743-1-APDownload protocol
IHC protocol for ACE antibody 24743-1-APDownload protocol
WB protocol for ACE antibody 24743-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Res

The AHCY-adenosine complex rewires mRNA methylation to enhance fatty acid biosynthesis and tumorigenesis

Authors - Kun Liao
View Article
mouseIHC

Kidney Int

Non-canonical Wnt/calcium signaling is protective against podocyte injury and glomerulosclerosis

Authors - Dong Zhou
View Article
mouseIHC

Adv Sci (Weinh)

Tailored Porous Bimetallic Nanozyme Platform for Full-Cycle Therapeutics of Intestinal Ischemia/Reperfusion.

Authors - Chenghao Qiu
View Article
ratWB

Phytomedicine

Fritillaria hupehensis cultivated under the canopy of Magnolia officinalis demonstrated superior anti-inflammatory and expectorant effects.

Authors - Xiaoyue Wang
View Article
mouseWB

Int J Biol Macromol

Angiotensin-converting enzyme inhibitory peptide IVGFPAYGH protects against liver injury in mice fed a high‑sodium diet by inhibiting the RAS and remodeling gut microbial communities

Authors - Lifang Zou
View Article
bovineWB

Int J Biol Macromol

TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.

Authors - Na Wang
View Article
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