Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, IMR-32 cells, SiHa cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88168-3-RR targets ACD in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag23021 Product name: Recombinant human ACD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC016904 Sequence: MPGRCQSDAAMRVNGPASRAPAGWTSGSLHTGPRAGRPRAQARGVRGRGLLLRPRPAKELPLPRKGGAWAPAGNPGPLHPLGVAVGMAGSGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDVGATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFRGTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFSLLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSESTSSNAGLSLSQLLDEMREDQEHQGALVCLAESCLTLEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCISENDQLILSSLGPCQRTQGPELPPPDPALQDLSLTL 相同性解析による交差性が予測される生物種 |
| Full Name | adrenocortical dysplasia homolog (mouse) |
| Calculated molecular weight | 58 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC016904 |
| Gene Symbol | ACD |
| Gene ID (NCBI) | 65057 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96AP0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACD (Adrenocortical Dysplasia Homolog), also known as TPP1 or POT1- and TIN2-Organizing Protein, is a core component of the telomeric Shelterin complex. It participates in telomere length maintenance, telomere structural protection, and regulation of genomic stability. Together with POT1, TIN2, TRF1, TRF2, RAP1, and TIN2, ACD assembles into the Shelterin complex, which coats telomeric DNA, suppresses DNA damage response and telomere fusion, and prevents chromosome ends from being recognized as DNA double-strand breaks. Mutations in the ACD gene cause Dyskeratosis Congenita, Hoyeraal-Hreidarsson syndrome, and bone marrow failure syndromes, and are closely associated with telomerase dysfunction.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACD antibody 88168-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |