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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ACAA1 Polyclonal antibody

ACAA1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12319

Synonyms

ACAA, ACAA1, Acetyl CoA acyltransferase, Beta ketothiolase, PTHIO, THIO

Validation Data Gallery

Applications:
  • IF Staining of U-251 using CL488-12319

    IF Staining of U-251 using CL488-12319

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 ACAA1 antibody (CL488-12319) at dilution of 1:200.

Tested Applications

Positive IF detected inU-251 cells
Planning an IHC experiment? We recommend our IHCeasy ACAA1 Ready-To-Use IHC Kit. ACAA1 primary antibody included.

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-12319 targets ACAA1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAA1 fusion protein Ag2972 相同性解析による交差性が予測される生物種
Full Name acetyl-Coenzyme A acyltransferase 1
Calculated molecular weight 424 aa, 44 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC011977
Gene symbol ACAA1
Gene ID (NCBI) 30
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACAA1 (acetyl-CoA acyltransferase 1) is an enzyme involved in lipid β-oxidation and provides substrates to the tricarboxylic acid (TCA) cycle, a critical step in cellular metabolism (PMID:33194642). ACAA1 is also a biomarker in type 2 diabetes (T2D), predicting the pre-diabetic metabolic signature in mouse models (PMID:25784953). Moreover, ACAA1 is highly expressed in TNBC, serving as a potential therapeutic target in ACAA1-high tumors and a predictive biomarker of resistance to CDK4/6 inhibitors for RB1-proficient patients (PMID:37129951).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ACAA1 antibody CL488-12319Download protocol
Standard Protocols
Click here to view our Standard Protocols