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Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using PE-conjugated ABHD5 Polyclonal antibody (PE-12201)

    FC experiment of mouse splenocytes using PE-12201

    1x10^6 mouse splenocytes were intracellularly stained with 0.1 ug PE-conjugated ABHD5 Polyclonal antibody (PE-12201)(red), or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of mouse thymocytes using PE-conjugated ABHD5 Polyclonal antibody (PE-12201)

    FC experiment of mouse thymocytes using PE-12201

    1x10^6 mouse thymocytes were intracellularly stained with 0.1 ug PE-conjugated ABHD5 Polyclonal antibody (PE-12201)(red), or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO Validated

PE-conjugated ABHD5 Polyclonal antibody

ABHD5 Polyclonal Antibody for ELISA
Cat No. PE-12201

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

ELISA

Synonyms

1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase domain-containing protein 5, CDS, CGI58, EC:2.3.1.51

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size/Concentration: 

-/ -

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Tested Applications

Positive FC (Intra) detected inmouse splenocytes, mouse thymocytes

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.1 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-12201 targets ABHD5 in ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABHD5 fusion protein Ag2840 相同性解析による交差性が予測される生物種
Full Name abhydrolase domain containing 5
Calculated molecular weight 349 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC021958
Gene Symbol ABHD5
Gene ID (NCBI) 51099
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WTS1
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).

Protocols

Product Specific Protocols
FC protocol for PE ABHD5 antibody PE-12201Download protocol
Standard Protocols
Click here to view our Standard Protocols