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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCG5 Polyclonal antibody (27722-1-AP)

    WB analysis using 27722-1-AP (same clone as 27722-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27722-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ABCG5 Polyclonal antibody (27722-1-AP)

    WB analysis using 27722-1-AP (same clone as 27722-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27722-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ABCG5 Polyclonal antibody (27722-1-AP)

    WB analysis using 27722-1-AP (same clone as 27722-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27722-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ABCG5 Polyclonal antibody (27722-1-AP)

    WB analysis of HepG2 using 27722-1-AP (same clone as 27722-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27722-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using ABCG5 Polyclonal antibody (27722-1-AP)

    WB analysis of mouse colon using 27722-1-AP (same clone as 27722-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27722-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ABCG5 Polyclonal antibody (27722-1-AP)

    WB analysis of mouse liver using 27722-1-AP (same clone as 27722-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27722-1-PBS in a different storage buffer formulation.

ABCG5 Polyclonal antibody, PBS Only

ABCG5 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 27722-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Sterolin 1, STSL

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27722-1-PBS targets ABCG5 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26844

Product name: Recombinant human ABCG5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-85 aa of NM_022436

Sequence: MGDLSSLTPGGSMGLQVNRGSQSSLEGAPATAPEPHSLGILHASYSVSHRVRPWWDITSCRQQWTRQILKDVSLYVESGQIMCIL

相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family G (WHITE), member 5
Calculated molecular weight 72 kDa
Observed molecular weight 68 kDa~72 kDa
GenBank accession numberNM_022436
Gene Symbol ABCG5
Gene ID (NCBI) 64240
RRIDAB_2880952
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H222
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ABCG5 belongs to the ABC transporter subfamily, whose members are "half-transporters" that require the formation of homodimers or heterodimers to function(PMID: 14504269). ABCG5 and ABCG8 form an obligate heterodimer that limits intestinal absorption and facilitates biliary secretion of cholesterol and phytosterols(PMID: 24252657). Mutations in ABCG5e may contribute to sterol accumulation and atherosclerosis(PMID: 15054092).

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