ABCG5 Polyclonal antibody

Name: ABCG5 Polyclonal antibody
Brand: Proteintech
SKU: 27722-1-AP

ABCG5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 27722-1-AP

Synonyms

ABCG5, Sterolin 1, STSL

Validation Data Gallery

Applications:

AllWBIHC

WB analysis using 27722-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 27722-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 27722-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 27722-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 27722-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 27722-1-AP (ABCG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 27722-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27722-1-AP (ABCG5 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 27722-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27722-1-AP (ABCG5 antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 27722-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Caco-2 cells, HepG2 cells, mouse colon tissue, mouse liver tissue, rat liver tissue
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 27 publications below
IHC	See 1 publications below

Product Information

27722-1-AP targets ABCG5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ABCG5 fusion protein Ag26844 相同性解析による交差性が予測される生物種
Full Name	ATP-binding cassette, sub-family G (WHITE), member 5
Calculated molecular weight	72 kDa
Observed molecular weight	68 kDa~72 kDa
GenBank accession number	NM_022436
Gene symbol	ABCG5
Gene ID (NCBI)	64240
RRID	AB_2880952
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ABCG5 antibody 27722-1-AP	Download protocol
IHC protocol for ABCG5 antibody 27722-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Diabetes Long Noncoding RNA lncRHL Regulates Hepatic VLDL Secretion by Modulating hnRNPU/ BMAL1/MTTP Axis. Authors - Xuan Shen View Article
human	WB	Cell Mol Gastroenterol Hepatol A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function. Authors - Dayan Sun View Article
human	WB	Mol Ther Nucleic Acids HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis. Authors - Zhi Zhang View Article
human	WB	J Cell Mol Med The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRα and PPARα. Authors - Ting Li View Article
rat	WB	Front Nutr Preparation of microgel co-loaded with nuciferine and epigallocatechin-3-gallate for the regulation of lipid metabolism Authors - Shengnan Zhu View Article
mouse,human	WB	Biochem Pharmacol Resveratrol enhances trans-intestinal cholesterol excretion through selective activation of intestinal liver X receptor alpha. Authors - Juan Pang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ABCG5 Polyclonal antibody

ABCG5 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 27722-1-AP

WB analysis using 27722-1-AP

WB analysis of HepG2 using 27722-1-AP

WB analysis of mouse colon using 27722-1-AP

WB analysis of mouse liver using 27722-1-AP

IHC staining of human liver using 27722-1-AP

IHC staining of human liver using 27722-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Long Noncoding RNA lncRHL Regulates Hepatic VLDL Secretion by Modulating hnRNPU/ BMAL1/MTTP Axis.

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.

The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRα and PPARα.

Preparation of microgel co-loaded with nuciferine and epigallocatechin-3-gallate for the regulation of lipid metabolism

Resveratrol enhances trans-intestinal cholesterol excretion through selective activation of intestinal liver X receptor alpha.