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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ABCB8 Polyclonal antibody (27158-1-AP)

    WB analysis of MCF-7 using 27158-1-AP (same clone as 27158-1-PBS)

    WB result of ABCB8 antibody (27158-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCB8 transfected MCF-7 cells. This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ABCB8 Polyclonal antibody (27158-1-AP)

    WB analysis using 27158-1-AP (same clone as 27158-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27158-1-AP (ABCB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ABCB8 Polyclonal antibody (27158-1-AP)

    IHC staining of mouse liver using 27158-1-AP (same clone as 27158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27158-1-AP (ABCB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ABCB8 Polyclonal antibody (27158-1-AP)

    IHC staining of mouse liver using 27158-1-AP (same clone as 27158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27158-1-AP (ABCB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using ABCB8 Polyclonal antibody (27158-1-AP)

    IF Staining of H9C2 using 27158-1-AP (same clone as 27158-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using ABCB8 antibody (27158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27158-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ABCB8 Polyclonal antibody, PBS Only

ABCB8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27158-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ABCB 8, ATP Binding Cassette Subfamily B Member 8, ATP-binding cassette sub-family B member 8, mitochondrial, M ABC1, MABC1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27158-1-PBS targets ABCB8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25935

Product name: Recombinant human ABCB8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 403-596 aa of BC141814

Sequence: TVQRSMANLSVLFGQVVRGLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVGQSGGGKTTVASLLERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAAREANAHEFITSFPEGYNTVVGERGTTLS

相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 8
Calculated molecular weight 735 aa, 80 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC141814
Gene Symbol ABCB8
Gene ID (NCBI) 11194
RRIDAB_3742217
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUT2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ABCB8 (ATP-binding cassette sub-family B member 8) is a protein located in the mitochondrial inner membrane and belongs to the ATP-binding cassette (ABC) transporter superfamily. It plays a crucial role in maintaining mitochondrial iron homeostasis and the maturation of cellular iron-sulfur cluster proteins. ABCB8 is essential for normal cardiac function by promoting the export of iron from mitochondria. Additionally, ABCB8 forms a complex with the mitochondrial KATP channel, protecting cells from oxidative stress damage. The loss of function or abnormal expression of ABCB8 is associated with various diseases, including cardiomyopathy and iron metabolism disorders.

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