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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ABCB8 Polyclonal antibody (27158-1-AP)

    WB analysis of MCF-7 using 27158-1-AP

    WB result of ABCB8 antibody (27158-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCB8 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using ABCB8 Polyclonal antibody (27158-1-AP)

    WB analysis using 27158-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27158-1-AP (ABCB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ABCB8 Polyclonal antibody (27158-1-AP)

    IHC staining of mouse liver using 27158-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27158-1-AP (ABCB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ABCB8 Polyclonal antibody (27158-1-AP)

    IHC staining of mouse liver using 27158-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27158-1-AP (ABCB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using ABCB8 Polyclonal antibody (27158-1-AP)

    IF Staining of H9C2 using 27158-1-AP

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using ABCB8 antibody (27158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  • KD/KO Validated

ABCB8 Polyclonal antibody

ABCB8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27158-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ABCB 8, ATP Binding Cassette Subfamily B Member 8, ATP-binding cassette sub-family B member 8, mitochondrial, M ABC1, MABC1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, MDA-MB-231 cells, mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inH9C2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27158-1-AP targets ABCB8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCB8 fusion protein Ag25935 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 8
Calculated molecular weight 735 aa, 80 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC141814
Gene Symbol ABCB8
Gene ID (NCBI) 11194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABCB8 (ATP-binding cassette sub-family B member 8) is a protein located in the mitochondrial inner membrane and belongs to the ATP-binding cassette (ABC) transporter superfamily. It plays a crucial role in maintaining mitochondrial iron homeostasis and the maturation of cellular iron-sulfur cluster proteins. ABCB8 is essential for normal cardiac function by promoting the export of iron from mitochondria. Additionally, ABCB8 forms a complex with the mitochondrial KATP channel, protecting cells from oxidative stress damage. The loss of function or abnormal expression of ABCB8 is associated with various diseases, including cardiomyopathy and iron metabolism disorders.

Protocols

Product Specific Protocols
WB protocol for ABCB8 antibody 27158-1-APDownload protocol
IHC protocol for ABCB8 antibody 27158-1-APDownload protocol
IF protocol for ABCB8 antibody 27158-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols