Validation Data Gallery

Filter: AllWBIHC

WB analysis using 22670-1-AP (same clone as 22670-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.
WB analysis of rat stomach using 22670-1-AP (same clone as 22670-1-PBS)
rat stomach tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.
WB analysis of mouse pancreas using 22670-1-AP (same clone as 22670-1-PBS)
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.
WB analysis of mouse stomach using 22670-1-AP (same clone as 22670-1-PBS)
mouse stomach tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 22670-1-AP (same clone as 22670-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 22670-1-AP (same clone as 22670-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 22670-1-AP (same clone as 22670-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 22670-1-AP (A4GNT antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 22670-1-AP (same clone as 22670-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 22670-1-AP (same clone as 22670-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.
IHC staining of rat stomach using 22670-1-AP (same clone as 22670-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.
IHC staining of rat stomach using 22670-1-AP (same clone as 22670-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 22670-1-AP (same clone as 22670-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 22670-1-AP (same clone as 22670-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

A4GNT Polyclonal antibody, PBS Only

A4GNT Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 22670-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:2.4.1.-, Alpha4GnT, Alpha-1,4-N-acetylglucosaminyltransferase

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22670-1-PBS targets A4GNT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18432 Product name: Recombinant human A4GNT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 76-340 aa of BC119639 Sequence: KIYPEWPVVFFMKGLTDSTPMPSNSTYPAFSFLSAIDNVFLFPLDMKRLLEDTPLFSWYNQINASAERNWLHISSDASRLAIIWKYGGIYMDTDVISIRPIPEENFLAAQASRYSSNGIFGFLPHHPFLWECMENFVEHYNSDIWGNQGPELMTRMLRVWCKLEDFQEVSDLRCLNISFLHPQRFYPISYREWRRYYEVWDTEPSFNVSYALHLWNHMNQEGRAVIRGSNTLVENLYRKHCPRTYRDLIKGPEGSVTGELGPGNK 相同性解析による交差性が予測される生物種
Full Name	alpha-1,4-N-acetylglucosaminyltransferase
Calculated molecular weight	340 aa, 39 kDa
Observed molecular weight	34-37 kDa
GenBank accession number	BC119639
Gene Symbol	A4GNT
Gene ID (NCBI)	51146
RRID	AB_11183768
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UNA3
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

A4GNT (alpha-1,4-N-acetylglucosaminyltransferase) catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans, forming a unique glycan structure, GlcNAcalpha1-->4Galbeta-->R. The gene is expressed with a bias in certain tissues, particularly in the stomach (RPKM 9.5) and duodenum (RPKM 0.9). Alpha4GnT has been implicated in O-glycan biosynthesis and is thought to have a role in preventing gastric cancer by inhibiting Helicobacter pylori infection and suppressing tumor-promoting inflammation.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22670-1-AP (same clone as 22670-1-PBS)

WB analysis of rat stomach using 22670-1-AP (same clone as 22670-1-PBS)

WB analysis of mouse pancreas using 22670-1-AP (same clone as 22670-1-PBS)

WB analysis of mouse stomach using 22670-1-AP (same clone as 22670-1-PBS)

IHC staining of human stomach cancer using 22670-1-AP (same clone as 22670-1-PBS)

IHC staining of human stomach cancer using 22670-1-AP (same clone as 22670-1-PBS)

IHC staining of human stomach using 22670-1-AP (same clone as 22670-1-PBS)

IHC staining of human pancreas cancer using 22670-1-AP (same clone as 22670-1-PBS)