• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

A4GNT Polyclonal antibody

A4GNT Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 22670-1-AP

Synonyms

A4GNT, alpha4GnT

Validation Data Gallery

Applications:
  • WB analysis using 22670-1-AP

    WB analysis using 22670-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse pancreas using 22670-1-AP

    WB analysis of mouse pancreas using 22670-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse stomach using 22670-1-AP

    WB analysis of mouse stomach using 22670-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of rat stomach using 22670-1-AP

    WB analysis of rat stomach using 22670-1-AP

    rat stomach tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 22670-1-AP

    IHC staining of human stomach cancer using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 22670-1-AP

    IHC staining of human stomach cancer using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 22670-1-AP

    IHC staining of human pancreas cancer using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 22670-1-AP

    IHC staining of human pancreas cancer using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat stomach using 22670-1-AP

    IHC staining of rat stomach using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of rat stomach using 22670-1-AP

    IHC staining of rat stomach using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of mouse stomach using 22670-1-AP

    IHC staining of mouse stomach using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 22670-1-AP

    IHC staining of human pancreas using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 22670-1-AP (A4GNT Antibody) at dilution of 1:50.

  • IHC staining of mouse stomach using 22670-1-AP

    IHC staining of mouse stomach using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human pancreas using 22670-1-AP

    IHC staining of human pancreas using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 22670-1-AP (A4GNT Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inK-562 cells, mouse pancreas tissue, mouse stomach tissue, rat stomach tissue, L02 cells
Positive IHC detected inhuman stomach cancer tissue, human pancreas cancer tissue, rat stomach tissue, mouse stomach tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22670-1-AP is A4GNT Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen A4GNT fusion protein Ag18432 相同性解析による交差性が予測される生物種
Full Name alpha-1,4-N-acetylglucosaminyltransferase
Calculated molecular weight 340 aa, 39 kDa
Observed molecular weight 34-37 kDa
GenBank accession numberBC119639
Gene symbol A4GNT
Gene ID (NCBI) 51146
RRIDAB_11183768
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for A4GNT antibody 22670-1-APDownload protocol
IHC protocol for A4GNT antibody 22670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols