Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.1 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-FcA98450 targets 6*His, His-Tag in FC (Intra) applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
unknow 相同性解析による交差性が予測される生物種 |
| Full Name | 6*His, His-Tag |
| Calculated molecular weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
| 激发激光 | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | HISTAG |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.