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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using 14-3-3 Monoclonal antibody (66061-1-Ig)

    WB analysis using 66061-1-Ig (same clone as 66061-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66061-1-Ig (14-3-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat brain tissue using 14-3-3 Monoclonal antibody (66061-1-Ig)

    IP experiment of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

    IP result of anti-14-3-3 (IP:66061-1-Ig, 4ug; Detection:66061-1-Ig 1:20000) with rat brain tissue lysate 1080 ug. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using 14-3-3 Monoclonal antibody (66061-1-Ig)

    IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using 14-3-3 Monoclonal antibody (66061-1-Ig)

    IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using 14-3-3 Monoclonal antibody (66061-1-Ig)

    IHC staining of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using 14-3-3 Monoclonal antibody (66061-1-Ig)

    IHC staining of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using 14-3-3 Monoclonal antibody (66061-1-Ig)

    IF Staining of HepG2 using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66061-1-Ig (14-3-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

14-3-3 Monoclonal antibody, PBS Only

14-3-3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66061-1-PBS
Clone No.2E10A3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

YWHAQ, 14-3-3 protein T-cell, 14-3-3 theta, 14-3-3 zeta, 14-3-3σ

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66061-1-PBS targets 14-3-3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6055

Product name: Recombinant human 14-3-3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-245 aa of BC056867

Sequence: MEKTELIQKAKLAEQAERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPIRLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDSAGEECDAAEGAEN

相同性解析による交差性が予測される生物種
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Calculated molecular weight 28 kDa
Observed molecular weight 31 kDa, 28 kDa
GenBank accession numberBC056867
Gene Symbol 14-3-3 theta
Gene ID (NCBI) 10971
RRIDAB_11042772
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP27348
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

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