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Recombinant Rat Lipocalin-2/NGAL protein (His Tag) (HPLC verified)

Species

Rat

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

His Tag

Activity

not tested

Cat no : Eg1022

Validation Data Gallery

  • Purity of Recombinant Rat Lipocalin-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Rat Lipocalin-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Rat Lipocalin-2/NGAL was greater than 90% as determined by SEC-HPLC.

    The purity of Rat Lipocalin-2/NGAL was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Rat Lipocalin-2 protein Gln21-Asn198 (Accession# P30152) with a His tag at the C-terminus.
GeneID 170496
Accession P30152
PredictedSize 21.6 kDa
SDS-PAGE 22-26 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond.

References:

1. Lim D, et al. (2021) CNS Neurosci Ther.27:883-94.
2. Kjeldsen L, et al. (1993) J Biol Chem.268:10425-32.
3. Devireddy LR, et al. (2005) Cell.123:1293-305.
4. Xing C, et al. (2014) Stroke.45:2085-92.
5. Asaf S, et al. (2023) Immunol Res.71:287-313.