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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

human IgD Polyclonal antibody

human IgD Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16489-1-AP

Synonyms

IGHD

Validation Data Gallery

Applications:
  • WB analysis using 16489-1-AP

    WB analysis using 16489-1-AP

    human plasma tissue were subjected to SDS PAGE followed by western blot with 16489-1-AP (human IgD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 16489-1-AP

    IHC staining of human tonsillitis using 16489-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16489-1-AP (human IgD antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgD antibody included
  • IHC staining of human tonsillitis using 16489-1-AP

    IHC staining of human tonsillitis using 16489-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16489-1-AP (human IgD antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgD antibody included
  • IF Staining of human tonsillitis using 16489-1-AP

    IF Staining of human tonsillitis using 16489-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using human IgD antibody (16489-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy human IgD Ready-To-Use IHC Kit. human IgD primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:3200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

16489-1-AP targets human IgD in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen human IgD fusion protein Ag9614 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant delta
Calculated molecular weight 573 aa, 63 kDa
Observed molecular weight 55-70 kDa
GenBank accession numberBC021276
Gene symbol IGHD
Gene ID (NCBI) 3495
RRIDAB_2878264
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. This antibody recognize the heavy chain (~70 kDa) of IgD.

Protocols

Product Specific Protocols
WB protocol for human IgD antibody 16489-1-APDownload protocol
IHC protocol for human IgD antibody 16489-1-APDownload protocol
IF protocol for human IgD antibody 16489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols