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CoraLite® Plus 488-conjugated ITPR1-specific Polyclonal antibody

ITPR1-specific Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19962

Synonyms

INSP3R1, IP3 receptor isoform 1, IP3R, IP3R 1, IP3R1, ITPR1, SCA15, SCA16, Type 1 InsP3 receptor

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated ITPR1-specific Polyc (CL488-19962)

    IF Staining of mouse brain using CL488-19962

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 ITPR1-specific antibody (CL488-19962) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated ITPR1-specific Polyc (CL488-19962)

    FC experiment of HepG2 using CL488-19962

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ITPR1-specific (CL488-19962) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inmouse brain tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19962 targets ITPR1-specific in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name inositol 1,4,5-triphosphate receptor, type 1
Calculated Molecular Weight 314 kDa
Observed Molecular Weight 290-300 kDa
GenBank Accession NumberNM_001099952
Gene Symbol ITPR1
Gene ID (NCBI) 3708
RRIDAB_2919168
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ITPR1-specific antibody CL488-19962Download protocol
FC protocol for CL Plus 488 ITPR1-specific antibody CL488-19962Download protocol
Standard Protocols
Click here to view our Standard Protocols