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CoraLite® Plus 488-conjugated CA12 Polyclonal antibody
CA12 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-15180
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-15180 targets CA12 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CA12 fusion protein Ag7107 |
Full Name | carbonic anhydrase XII |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 44 kDa, 75-80 kDa |
GenBank Accession Number | BC000278 |
Gene Symbol | CA12 |
Gene ID (NCBI) | 771 |
RRID | AB_2919111 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 CA12 antibody CL488-15180 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |