×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NOC2L Recombinant monoclonal antibody (88267-1-RR)

    WB analysis using 88267-1-RR (same clone as 88267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88267-1-RR (NOC2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88267-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NOC2L Recombinant monoclonal antibody (88267-1-RR)

    WB analysis using 88267-1-RR (same clone as 88267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88267-1-RR (NOC2L antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using NOC2L Recombinant monoclonal antibody (88267-1-RR)

    IHC staining of human placenta using 88267-1-RR (same clone as 88267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 88267-1-RR (NOC2L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88267-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NOC2L Recombinant monoclonal antibody (88267-1-RR)

    IF Staining of U2OS using 88267-1-RR (same clone as 88267-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOC2L antibody (88267-1-RR, Clone: 260756G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 88267-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03442-1

    Cytometric bead array sample test of MP03442-1

    The mean NOC2L concentration was determined to be 26.5 ng/mL in Raji cell extract based on a 1.0 mg/mL extract load, 26.8 ng/mL in HT-1080 cell extract based on a 1.0 mg/mL extract load, 20.3 ng/mL in U2OS cell extract based on a 1.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP03442-1

    Cytometric bead array standard curve of MP03442-1

    Cytometric bead array standard curve of MP03442-1, NOC2L Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88267-1-PBS. Detection antibody: 88267-2-PBS. Standard: Ag26952. Range: 0.156-20 ng/mL.

  • Affinity of 88267-1-RR

    Affinity of 88267-1-RR

    Biolayer interferometry (BLI) kinetic assays of 88267-1-RR against Human NOC2L were performed. The affinity constant is 0.413 nM.

NOC2L Recombinant monoclonal antibody, PBS Only (Capture)

NOC2L Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 88267-1-PBS
Clone No.260756G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

NIR, NOC2-like protein, Novel INHAT repressor, Nucleolar complex protein 2 homolog, Protein NOC2 homolog

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

88267-1-PBS targets NOC2L as part of a matched antibody pair:

MP03442-1: 88267-1-PBS capture and 88267-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26952

Product name: Recombinant human NOC2L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-202 aa of BC003555

Sequence: MAAAGSRKRRLAELTVDEFLASGFDSESESESENSPQAETREAREAARSPDKPGGSPSASRRKGRASEHKDQLSRLKDRDPEFYKFLQENDQSLLNFSDSDSSEEEEGPFHSLPDVLEEASEEEDGAEEGEDGDRVPRGLKGKKNSVPVTVAMVERWKQAAKQRLTPKLFHEVVQAFRAAVATTRGDQESAEANKFQVTDSA

相同性解析による交差性が予測される生物種
Full Name nucleolar complex associated 2 homolog (S. cerevisiae)
Calculated molecular weight 749 aa, 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC003555
Gene Symbol NOC2L
Gene ID (NCBI) 26155
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3T9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).

||
New chat

Able™

正在加载，请稍候...